Head of the Melbourne Mass Spectrometry and Proteomics Facility To access the mass spectrometers contact: Follow the link to Pubmed to see a list of publications.Three Shimadzu Prep HPLC with UV and MS detection.Two Agilient prep scale HPLC systems with UV and MS detection.Two Agilent HPLC systems for sample preparation.Deadbox for proteomic sample preparation.The laboratory also has a number of general equipment items for use: Trajan /Waters HDX system ( robotic HDX platform).Agilent LC/ 8900 ICP-QQQ mass spectrometer.Agilent 6520 LC/ESI-TOF mass spectrometer.Thermo NanoLC/ OrbiTRAP Eclipse mass spectrometer.2 x Thermo NanoLC/ Q Exactive Plus Mass spectrometer.2 x Thermo NanoLC/ OrbiTrap Fusion Lumos.They start to redesign how they do things in order to take advantage of new workflows and more precise answers – why spend hours on an SDS-PAGE gel for a recombinant protein when in 15 minutes you can confirm its primary structure with an EXACT mass on the full-length protein? Mass Spectrometers We frequently find that users start very simple but as they get more experience they branch out to ever more complex and imaginative uses for the technology. The greatest benefit of using our facility is that you learn how to do it yourself. The only limit is that as a shared facility you cannot monopolize or run samples in preference to anyone else. Like a gym membership you can run as many samples as you like during your subscription. A laboratory head will pay the subscription cost ($7600 for University of Melbourne) and then every laboratory member can access the platform. The facility access cost is highly subsidized by the University of Melbourne and is charged on a quarterly, or yearly basis. Our costs are based on a membership structure. We have a fleet of eight mass spectrometers and we make it as easy as possible for you to learn as much or as little as you like about the actual instrumentation. We have more than 300 users from more than 70 different labs all over Victoria and Interstate as evidence that this approach is very effective. Perform both labelled and label-free quantitation experiments.Immune-precipitation of protein complexes, and.Characterize intact recombinant proteins by mass,.We teach you to perform your own mass spectrometry and proteomics experiments using our instrumentation.Īll advice on the planning, execution, and analysis of your samples is included in the cost. The Melbourne Mass Spectrometry and Proteomics Facility provides academic and industry researchers access to specialist mass spectrometry and proteomic equipment and expertise.
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